한국수산과학회

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  • pISSN : 2234-8395
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  • eISSN : 2234-1757

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kfas, vol. 50, no. 4, pp.447-452, August, 2017
DOI. https://doi.org/10.5657/KFAS.2017.0447

Molecular Identification of a Sea Anemone (Cnidaria: Anthozoa:
Actiniaria) Obtained in Gijang, Busan
부산 기장에서 채집된 말미잘의 분자생물학적 방법을 이용한 동정

유상준·김도형*
부경대학교 수산생명의학과

In this study, we tried to identify a sea anemone collected from the coast of Gijang, Busan. The anemone was morphologically
similar to species belonging to the genus Anthopleura, but its morphological characteristics did not
allow for confirmed identification to species level. Multiple genes from mitochondrial cytochrome oxidase III, 12S
and 16S rRNA, and nuclear 18S and 28S rRNA, were amplified for multilocus sequence typing (MLST) analysis using
genomic DNA extracted from the sampled anemone and a different primer set. Based on the MLST analysis, the
anemone obtained in this study was identified as Anthopleura artemisia. Also, the sequence of internal transcribed
spacer-2 was most closely related to A. artemisia, indicating that this single region might be useful for anemone identification.
This study shows significance of molecular identification for sea anemones, and will be helpful in studies
of sea anemone identification using genotyping-by-sequencing.

Keyword : Sea anemone, Actiniaria, Molecular identification, Anthopleura artemisia, Multilocus sequence typing

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