한국수산과학회

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  • pISSN : 2234-8395
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  • eISSN : 2234-1757

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kfas, vol. 52, no. 2, pp.141-148, April, 2019
DOI. https://doi.org/10.5657/KFAS.2019.0141

Effect of Fish Meal Replacement on Insulin-like Growth Factor-I Expression
in the Liver and Muscle and Implications for the Growth of
Olive Flounder Paralichthys olivaceus
사료의 어분함량대체가 넙치(Paralichthys olivaceus)의 간과 근육 내 인
슐린유사성장인자의 발현과 체성장에 미치는 영향

박수진·문지성·서진송·남택정1·이경준2·임상구3·김강웅4·이봉주3·허상우3·최윤희*
부경대학교 수산생물학과, 1부경대학교 식품영양학과, 2제주대학교 해양생명과학과, 3국립수산과학원 사료연구센터,4국립수산과학원 양식관리과

This study examined the effect of insulin-like growth factor (IGF)-I expression in the liver and muscle on the growth
of Paralichthys olivaceus fed diets low in fish meal. A feeding experiment was conducted at Jeju National University,
Jeju Island, Korea. Groups of P. olivaceus (total initial weight: 200 g) were maintained for 20 weeks on one
of five experimental diets containing different proportions of fish meal. Diets containing 0%, 20%, 30%, 40%, and
50% fish meal were labeled FM0, FM20, FM30, FM40, and FM50, respectively. Fish growth was observed every 4
weeks during the feeding experiment, and plasma and liver and muscle tissues were sampled. Plasma IGF-I levels
were analyzed using an ELISA kit. The mechanism of IGF-I receptor signaling was examined using immunoblotting
and reverse transcription-polymerase chain reaction. The greatest total weight increase was observed in the FM30
group. In parallel, plasma levels of IGF-I and IGF-binding protein were highest in the FM30 group, and mRNA and
protein expression were also significantly higher in this group. The first step in the IGF-I signaling pathway, tyrosinephosphorylation
checking, occurred smoothly until 20 weeks. These results suggest that a dietary ratio of 30% fish
meal best promotes growth in this species. The IGF-I signaling pathway in the liver and muscle is associated with
growth in P. olivaceus.

Keyword : Olive flounder, Insulin-like growth factor I, Growth, Low-fish meal diet

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