한국수산과학회

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  • pISSN : 2234-8395
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  • eISSN : 2234-1757

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kfas, vol. 53, no. 1, pp.123-131, February, 2020
DOI. https://doi.org/10.5657/KFAS.2020.0123

Cytoprotective Effect of a Neutrase Enzymatic Hydrolysate Derived
from Korea Pen Shell Atrina pectinata Against Hydrogen Peroxide
-Induced Oxidative Damages in Hepatocytes
산화적 손상에 대한 키조개(Atrina pectinata) 효소 가수분해물의 간세포
보호 효과

한의정1,2·신은지2·김기웅3·안긴내2,3·배태진2,3*
유)난타 5000, 2전남대학교 식품공학·영양학과, 3전남대학교 해양바이오식품학과

In this study, we investigated the protective effects of a Neutrase enzymatic hydrolysate derived from Korea pen shell
Atrina pectinata (APN) against hydrogen peroxide (H2O2)-induced oxidative damage in hepatocytes. First, we confirmed
that APN has antioxidant activities by scavenging 2,2-azino-bis (3-ethylbenzthiazoline)-6-sulfonic acid radical
(ABTS+) and H2O2 and increasing oxygen radical absorbance capacity (ORAC) value. Also, the treatment of APN
increased the cell viability by reducing the intracellular reactive oxygen species (ROS) production in H2O2-stimulated
hepatocytes. In addition, APN decreased the sub-G1 DNA contents and the apoptotic body formation increased by
H2O2 stimulation. Moreover, APN modulated the protein expression of apoptosis related molecules (Bcl-2, Bax and
p53) by suppressing the activation of nuclear factor NFkB and ERK/p38 signaling in H2O2-stimulated hepatocytes.
Furthermore, APN led to the activation of Nrf2/HO-1signaling known as antioxidant systems. These results suggest
APN protects hepatocytes against oxidative damages caused by H2O2 stimulation.

Keyword : Atrina pectinate, Hydrogen peroxide, Oxidative damages, Hepatocytes

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