한국수산과학회

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kfas, vol. 54, no. 4, pp.404-410, August, 2021
DOI. https://doi.org/10.5657/KFAS.2021.0404

Verification of Analytical Method of Azaspiracid Toxins in Shellfish and Tunicates by Liquid Chromatography-Tandem Mass Spectrometry LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry)를 이용한 패류 및 피낭류 중 아자스필산 분석법의 유효성 검증

조성래*·정상현·박큰바위1 ·윤민철1 ·김동욱1 ·손광태1 ·하광수
국립수산과학원 남동해수산연구소, 1 국립수산과학원 식품위생가공과

Although, mouse bioassay for the monitoring of azaspiracids (AZAs) toxins in shellfish has been used previously, the reported method has low sensitivity and it is time-consuming. Recently, there is an interest in the quantitative analysis of AZAs using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The purpose of this study is to verify the simultaneous analysis of AZAs in shellfish and tunicate in Korea using LC-MS/MS. To validate the method, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, and repeatability were determined. All standard compounds were analyzed within 7 min. The correlation coefficients (R2 ) of the standard solution was higher than 0.9995 (within the range of 0.8-10.0 μg/L). The LODs and LOQs of AZAs in shellfish were 0.08-0.16 μg/kg and 0.23-0.50 μg/kg, respectively. The accuracy and precision of the method for determining AZAs in shellfish were 87.1-93.0% and 1.23-4.91%, respectively. Consequently, the verified LC-MS/MS method is suitable to analyze AZAs in shellfish and tunicates in Korea.

Keyword : Azaspiracid toxin, LC-MS/MS, Validation, Shellfish, Tunicate

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