한국수산과학회

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kfas, vol. 54, no. 5, pp.652-659, October, 2021
DOI. https://doi.org/10.5657/KFAS.2021.0652

Assessment of Microorganism-derived Adjuvants for Scuticociliate
Miamiensis avidus Vaccine
스쿠티카충Miamiensis avidus 주사백신용 미생물유래 면역보조제의 평가

정명화·정성주1*
한서대학교 해양바이오수산생명의학과, 1전남대학교 수산생명의학과

Microorganism-derived compounds, such as peptidoglycan, lipoteichoic acid, and β-glucan were supplemented in the
scuticociliate Miamiensis avidus (M. avidus) vaccine to verify the specify component contribution to the adjuvant
effect. Vaccine was formulated with the inactivated M. avidus antigen (YS2, 4.44×105 cells/fish) in combination with
either peptidoglycan (10 μg and 100 μg/fish), lipoteichoic acid (5 μg and 50 μg/fish), or β-glucan (10 μg and 100 μg/
fish). Olive flounder injected with peptidoglycan supplemented vaccine (10 μg and 100 μg/fish) exhibited significant
protection, and the relative percent survival (RPS) was 55% and 65% at 4 weeks post vaccination (wpv), respectively,
at the corresponding doses. The vaccine groups with added lipoteichoic acid (5 μg and 50 μg/fish) exhibited RPS of
40% and 5%, respectively. Additionally, the group with added β-glucan (100 μg/fish) exhibited RPS of 35%, but no
effect was observed in the group with added 10 μg/fish β-glucan. At 8 wpv, olive flounder injected with peptidoglycan
and lipoteichoic acid supplemented vaccines exhibited protection with RPS range of 11/11% and 5/21%, respectively,
at the respective doses. M. avidus vaccine containing 10 μg and 100 μg/fish of β-glucan exhibited the RPS of 32% and
37%, respectively. Conclusively, peptidoglycan contributed in high protection of the M. avidus vaccine, and thus, it
can be used as an effective adjuvant in the M. avidus vaccine.

Keyword : Miamiensis avidus, Vaccine, Adjuvant, Peptidoglycan, Lipoteichoic acid

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