한국수산과학회

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kfas, vol. 54, no. 5, pp.691-697, October, 2021
DOI. https://doi.org/10.5657/KFAS.2021.0691

Development of a Simultaneous PCR Assay for Acute Hepatopancreatic
Necrosis Disease (AHPND) and Enterocytozoon hepatopenaei (EHP) in
Penaeus vannamei
흰다리새우(Penaeus vannamei)에서 급성간췌장괴사병(Acute Hepatopancreatic
Necrosis Disease)과 새우미포자충(Enterocytozoon
hepatopenaei)의 PCR 동시 진단법 개발

전혜진·이초롱·김범근·김수미1·장광일1·이강윤2·권혜민3·한지은*
경북대학교 수의학과, 1국립수산물품질관리원 수산방역과, 2군산대학교 해양과학대학, 3한국생명공학연구원 감염병연구센터

Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (VpAHPND) and Enterocytozoon hepatopenaei
(EHP) are the two most important pathogens in shrimp aquaculture and they have caused enormous losses
to the shrimp industry worldwide. In ponds, the major target organ for the two pathogens is the hepatopancreas,
and infection with EHP is a known potential risk factor for VpAHPND infection. This study aimed to develop a PCR
(polymerase chain reaction)-based diagnostic method for simultaneously detecting VpAHPND and EHP. The newly
developed PCR diagnostic method could be used to test various samples, such as seawater, shrimp, and feces. The
diagnostic method exhibited high sensitivity and specificity for both pathogens. This will help reduce the potential
economic losses that may have been caused by the two major shrimp pathogens, VpAHPND and EHP, and will allow for
the efforts and time spent combatting them to be dedicated elsewhere.

Keyword : Acute hepatopancreatic necrosis disease, Diagnosis, Enterocytozoon hepatopenaei, Peneaus (Litopenaeus) vannamei, Shrimp

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