한국수산과학회

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kfas, vol. 54, no. 6, pp.927-937, December, 2021
DOI. https://doi.org/10.5657/KFAS.2021.0927

Screening and Purification of a Novel Antibacterial Peptide, cgCAFLP,
Against Skin Pathogens from the Extract of the Pacific Oyster
Crassostrea gigas from Buan in Korea
부안산 참굴(Crassostrea gigas) 추출물로부터 피부 상재균에 대한 새로운
항균 펩타이드, cgCAFLP의 탐색 및 정제

이지은·서정길*
국립군산대학교 해양과학대학 식품생명공학전공

This study was performed to screen the antimicrobial activities of the extract from the Pacific oyster Crassostrea gigas
against skin pathogens and to purify the relevant antibacterial peptide. The acidified extract showed potent antibacterial
activities against gram-positive and gram-negative bacteria but showed no activity against Candida albicans and
no significant cell toxicity. Among acne-causing pathogens, the acidified extract showed potent antibacterial activity
only against Staphylococcus aureus, and its antibacterial activity was completely abolished by treatment with trypsin
or chymotrypsin, and was inhibited by salt treatment. The acidified extract showed strong DNA-binding ability but
did not show bacterial membrane permeabilizing ability. Based on antimicrobial activity screening and cytotoxic
effects, a novel antibacterial peptide was purified from the acidified gill extract using solid-phase extraction, cationexchange,
and reversed-phase HPLC. The resulting peptide had a molecular weight of 4800.8 Da and showed partial
sequence homology with the carbonic anhydrase 4 (CA4) protein in the hard-shelled mussel. Overall, we purified a
novel antibacterial peptide, named cgCAFLP, which is related to carbonic anhydrase 4 (CA4) protein, against skin
pathogens. Our results suggest that the Pacific oyster extract could be used as an additive to control some acne-related
skin pathogens (S. aureus).

Keyword : Crassostrea gigas, Skin pathogens, Antibacterial peptide, Purification, cgCAFLP

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