plSSN : 0374-8111 | elSSN : 2287-8815
plSSN : 0374-8111 | elSSN : 2287-8815
plSSN : 0374-8111elSSN : 2287-8815
이민지1,2·방단비1,2·신성수1,2·강윤자2*
1국립수산과학원 기후환경자원과, 2전남대학교 해양융합과학과
The reactive oxygen species (ROS) generated by harmful algal bloom species (HABs) have detrimental effects on aquaculture organisms. The destruction of fish gill cells upon exposure to HABs suggests that the internally generated ROS in HABs can influence the external environments. Therefore, using four representative HABs (Alexandrium affine, Chattonella marina, Karenia mikimotoi, Margalefidinium polykrikoides), the internal and external changes in ROS concentrations during their growth stages were investigated using fluorescence staining methods. The results showed that the concentrations of H2O2 and O2- produced by A. affine were low, while H2O2 from M. polykrikoides was mainly detected internally through the experiments, but O2- was not detected. High concentrations of H2O2 and O2- were observed in K. mikimotoi during the death phase, with weak external O2- concentrations. For C. marina, known to produce a large amount of ROS, H2O2 was most strongly observed internally during the exponential phase, while weak O2- concentrations were measured externally from the stationary phases. Collectively, our results highlight that ROS concentrations and internal/external distributions were a function of HABs and growth stages. The differences not only indicate the potential allelopathic mechanisms proliferating harmful algal blooms, but also suggest the potential impacts of ROS on aquaculture organisms.
HABs, ROS, Phytoplankton, Aquaculture organisms, Allelopathy