plSSN : 0374-8111 | elSSN : 2287-8815
plSSN : 0374-8111 | elSSN : 2287-8815
plSSN : 0374-8111elSSN : 2287-8815
Development and Characterization of Polymorphic Microsatellite Markers in Goniistius zonatus Using Next Generation Sequencing
동춘매·이미난·노은수·박진우1·김군도2·황인준·강정하·정효선*
국립수산과학원 생명공학과, 1국립수산과학원 아열대수산연구소, 2국립부경대학교 미생물학과
This study aimed to analyze the genetic diversity of Goniistius zonatus by developing microsatellite markers and evaluating their population genetic characteristics. Among a total of 188,476 genome contigs, microsatellite sequences were detected in 39,127 contigs, which contained a total of 48,789 candidate microsatellite markers. After removing redundant simple sequence repeat (SSR) motifs, 34,117 candidate markers were initially selected. Subsequently, polymorphism analysis, including the presence of insertions and deletions (InDels) within the SSR marker regions, resulted in the final selection of 3,648 markers. Among these 3,648 markers, 146 microsatellite markers were initially chosen based on the type of repeat motif, primer size, and amplification product location. PCR amplification and fragment size evaluation further refined the selection to 20 markers. The genetic characteristics of the Goniistius zonatus population were analyzed using the 20 selected microsatellite markers. The mean number of effective alleles was 9, ranging from 5 to 15. The observed heterozygosity (HO) and expected heterozygosity (HE) were found to be 0.755 and 0.766, respectively. The polymorphic information content (PIC) values ranged from 0.546 to 0.861, with an average of 0.722, indicating that these markers provide sufficient individual identification power for accurately analyzing the genetic diversity of the Goniistius zonatus population.
Goniistius zonatus, Next generation sequencing, Microsatellite markers, Genetic variability