plSSN : 0374-8111 | elSSN : 2287-8815
plSSN : 0374-8111 | elSSN : 2287-8815
plSSN : 0374-8111elSSN : 2287-8815
시베리아 철갑상어(Acipenser baerii) 부화 자어에서 미세주입 조건의 검증과초기 선천면역 마커 유전자의 발현 반응
김은정·남윤권*
국립부경대학교 수산생명과학부 양식응용생명과학전공
Hatching mark the transition of oviparous fish embryos from protection within egg envelope to direct exposure to aquatic microorganisms. In sturgeons, this occurs at the beginning of an extended yolk-dependent prelarval stage, when immune organs remain underdeveloped. However, the inducible innate defence capacity of newly hatched sturgeon prelarvae remains poorly characterized. This study examined acute immune-related transcriptional responses in newly hatched Siberian sturgeon Acipenser baerii prelarvae using microinjection-based stimulation model. Handling, anesthesia, non-injection, and saline injection produced no consistent changes in basal hamp or il1b expression, validating saline injection as a procedural control. Both LPS and poly (I:C) triggered rapid hamp upregulation, stronger at 6 h than 12 h post injection, though not strictly dose-proportional. Live bacterial injection showed stimulus-dependent patterns: Pathogenic Aeromonas hydrophila induced approximately 15 fold hamp induction at 6 h post injection, and transiently elevated il1b. In contrast, non-pathogenic Bacillus subtilis produced expression patterns comparable to those of saline controls. Survival observations supported the biological distinction between the bacterial treatments. These findings suggest that newly hatched Siberian sturgeon prelarvae can initiate selected antimicrobial and inflammatory gene responses despite limited immune organ differentiation.
Siberian sturgeon, Hatching, Microinjection conditions, Innate immune response